From March to June 2024, Alicia Wenghöfer, BSc, Research Technologist, carried out an internship at the National Institutes of Health (NIH) in Maryland, USA, as part of the Global Parkinson´s Genetics Program GP2. Within her 3-month stay, Alicia learned new methods of processing human DNA samples and generating long-read sequencing data to better understand the genetic architecture of Parkinson-related disorders.
At NIH, Alicia had the opportunity to develop a protocol called ‘Processing frozen archival human DNA samples for population-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP' that describes the processing of archival human DNA samples into long-read sequencing DNA data. This protocol facilitates the comparison between generated data of fresh-extracted DNA and archived-frozen DNA, especially across different laboratories.
https://www.protocols.io/view/processing-frozen-archival-human-dna-samples-for-l-5jyl82morl2w/v1